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Literature summary extracted from

  • Wu, C.; Kawasaki, K.; Ogawa, Y.; Yoshida, Y.; Ohgiya, S.; Ohmiya, Y.
    Preparation of biotinylated Cypridina luciferase and its use in bioluminescent enzyme immunoassay (2007), Anal. Chem., 79, 1634-1638.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.6 expressed in Saccharomyces cerevisiae Cypridina noctiluca

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.12.6 63000
-
 biotin-CLuc, SDS-PAGE Cypridina noctiluca

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.6 Cypridina noctiluca
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.6 Ni affinity column chromatography, SA beads column chromatography, monomeric avidin column chromatography, and TSK G300SW gel filtration Cypridina noctiluca

Storage Stability

EC Number Storage Stability Organism
1.13.12.6 4°C, PBS buffer containing 0.1 M potassium phosphate (pH 7.2) and 0.15 M NaCl, 2 months, without significant loss of activity Cypridina noctiluca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.6 Cypridina luciferin + O2
-
 Cypridina noctiluca oxidized Cypridina luciferin + CO2 + hv
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.6 CLuc
-
 Cypridina noctiluca
1.13.12.6 Cypridina luciferase
-
 Cypridina noctiluca

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.12.6 4
-
 The biotinylated luciferase is stable when stored at 4°C. Cypridina noctiluca